Prepare Substrate
30 minutesMix and pasteurize bulk substrate, prepare monotub with liner and air exchange holes.
Field context
This workflow is part of 3 niche fields
Complete guide for monotub tekk setup — step-by-step workflow, tools, checklist, and expert tips to get started.
Mix and pasteurize bulk substrate, prepare monotub with liner and air exchange holes.
Calculate fresh air exchange frequency, relative humidity targets, and colonization timeline per stage.
Mix colonized grain spawn into bulk substrate, level surface, and seal with filtered lid.
Track colonization progress, introduce FAE to trigger pinning, and maintain fruiting conditions.
Size fan airflow for monotub fresh air exchange during pinning and fruiting.
Calculate humidity dome or tub cover dimensions for target RH.
Set indirect light cycle duration for fruiting body development.
Estimate flush yield from bulk substrate volume.
Key benchmarks for monotub tekk setup.
| Stage | FC % | FAE |
|---|---|---|
| Colonization | 95+ | Minimal |
| Pinning | 90-95 | 2× daily |
| Fruiting | 85-92 | 4× daily |
Verify local laws before acquiring spores or spawn — penalties vary widely by jurisdiction.
Introducing fresh air too early before full colonization invites contamination through exposed substrate.
Disposable tub liners cost pennies and eliminate scrubbing between flushes.